The time related to genome sequencing depends on the genome length and the number of base pairs involved. The base pairs in DNA that are repetitive are difficult to map and analyze and hence, sequencing takes a longer period. Genome sequencing does not allow sequencing completely without any error as sequencing genome is not a sensitive process. For plants, the sequences from organelles like chloroplast, mitochondria are also mapped to sequence the genome fully. This is done to sequence the whole plant at once.
The genome project is a useful process as it helps to determine the whole genome of a particular organism, including eukaryotes. In humans, DNA sequencing is a limited process for the genome project as DNA forms a small proportion in the human genome. Moreover, the human genome includes several non-coding sequences, also known as junk DNA, the genome project also identifies those DNA sequences along with coding DNA. The genome project helps to predict the location of a particular gene in a genome. The project also provides information regarding the particular function linked with a particular gene. Moreover, map particular mRNA and defines its role in protein formation.
The target DNA sequences are separated and then put back in the same DNA by the process called genome assembly. The large DNA sequences are divided into smaller sequences and then processed using sequencing machines. The machines using advanced software can read more than 1000 nucleotides (adenine, guanine, cytosine, and thymine) at once. The software will detect repeating pairs of base pairs and then map the sequence and the process continues again. These pairs are known as repeats and are present in large numbers in a large genome including plants and animals.
Software like Short Oligonucleotide Analysis Package can detect and analyze large genome sequences in a short period. The results are more sensitive and specific. It can detect SNPs and alignment of the human genome. Gene annotation defines the information associated with the DNA sequence. It was created in the early 1980s with the help of molecular bioinformatics.
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